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药用原辅包质量审计、注册及供应；GMP 质量体系咨询；药物制剂研发技术支持和信息服务 各位专家、同行:
第三届“亚洲药剂学论坛(3rd Asian Symposium on Pharmaceutical Science and Technology,ASPST 2014)”暨第三届“亚洲药物制剂科学杂志(Asian Journal of Pharmaceutical Sciences, AJPS)编委会扩大会议”将于2014年5月25-27日在沈阳召开。届时,20余名来自亚洲以及欧美国家和地区的AJPS编委会成员和药剂学专家将受邀作大会报告,介绍国内外制剂科学与技术领域最前沿的研究进展和科研成果。会议还将广泛征集国内外著名专家学者的学术论文,就新型给药系统、工业药剂学、物理药剂学、粉体学、生物药剂学、制剂机械技术、药用辅料等方面的新进展、新动态和新成果进行深入交流与探讨。相信此次盛会的召开将为药剂学同行提供一个交流与合作的平台,推动亚洲药剂学的蓬勃发展。现诚挚邀请国内外从事药物制剂研究、开发的专业人员参加会议。
AJPS(ISSN,1818-0876)是由沈阳药科大学和出版商Elsevier联合出版、国内外公开发行的全英文双月刊,从2006年开始正式出版。在
一、会议时间:2014年5月25~27日, 5月24日报到
二、会议地点:沈阳万达文华酒店
三、会议内容:
1.成立第三届AJPS编委会,讨论如何发展AJPS
2.交流最新研究进展
3.参观沈阳药科大学
会议主题:药物制剂新技术与新剂型
会议专题:
1. 新剂型研究(口服缓/控释给药系统、靶向药物传递系统、微粒载体给药系统,纳米药物传递系统及生物大分子药物新型给药系统)
2、提高难溶性或难吸收药物生物利用度的对策
3.药用辅料在新剂型及仿制药品中的应用
4.粒子的加工技术与设备
5.药物制剂设备的开发与应用
6.提高仿制品质量的对策(辅料、新技术、新设备等)
7.医院药学及医院药物制剂
四、会议形式:
1. 交流方式:大会报告、墙报交流、展示(产品)等。
2. 交流语言:
5月26-27日普通报告:英语或汉语
3.报告时间:邀请报告:30分钟;普通报告:20分钟
五、征文要求:
1.未在国内外学术刊物上公开发表过的相关领域的论文,论文具有科学性、先进性和创新性。
2.论文用英文撰写,论文格式及模板请参见附录2和3。
3.投稿方式:论文以Word文档或PDF文件格式,发送电子邮件至 qkgjb2006@126.com 或ajps2006@163.com。稿件成功投稿后,将收到“论文收到”的回复,如果未收到,请致电AJPS编辑部查证(024-23986079)。邀请报告论文限4页,研究报告论文限2页。
4.口头报告的论文由编委会审查后择优录取,并于第二轮通知予以公布。会议征集到的优秀论文将被推荐在AJPS上发表。
6. 稿件截止日期:
六、收费标准:
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中国参会者 |
1800CNY |
2100 CNY |
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中国学生 |
800 CNY |
980 CNY |
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外国参会者 |
300US$ |
350US$ |
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外国学生 |
150US$ |
175US$ |
注:1. 注册费含论文集、茶歇以及会议期间(24日晚-26日晚)的中、晚餐。住宿和早餐自理。
2. AJPS 编委及审稿人免注册费,组委会提供住宿。
七、会议赞助和广告:
凡赞助本次会议的生产厂商,包括仪器设备生产厂、药用辅料和药品生产厂等,会场报告30分钟赞助费为5万元人民币,会场报告20分钟赞助费为3.5万元人民币。午餐休息时间报告30分钟者(自选)赞助费为3万元人民币,展台展示赞助费为2万元人民币,大会报告和分会场报告的企业均可免3人会务费和免费的墙报和展台使用(从会议开始到结束),展台展示可免2人会务费,所有赞助者食宿自理。
八、会议学分:
会议授予中国药学会药剂学专业委员会二类学分8分。
九、参会报名与联系方式:
以传真或电子邮件方式提交第三届“亚洲药剂学论坛”报名回执表(见附录1)至AJPS编辑部或“亚洲药剂学论坛”筹备组。报名截止日期为
未投稿者也可以报名参会。
地 址:沈阳市文化路103号,沈阳药科大学20#信箱
电 话/传 真:024-23986079 E-mail:qkgjb2006@126.com,ajps2006@163.com
联系人:凌桂霞 Tel:024-23986079 E-mail: ajps2006@163.com
毛世瑞 Tel:024-23986358 E-mail: maoshirui@vip.sina.com
何仲贵 Tel: 024-23986321 E-mail: hezhongui@vip.163.com
十、组织:
主办单位:中国药学会、亚洲药学联盟会、国际控释协会(CRS)中国分会
沈阳药科大学 AJPS编委会
承办单位:沈阳药科大学
组织委员会:名誉主席:永井恒司
主 席:何仲贵、竹内洋文、Paul W.S.Heng
副主席:毛世瑞
组织成员:“亚洲药剂学论坛”筹备组及亚洲各国以及其他国家和地区的编委。
十一、汇款方式:
1.请于
2.汇款单位名称:沈阳药科大学 (请在附言中标明“第三届亚洲药剂学论坛”)。
3.开户行:沈阳建设银行通汇支行,账 号:21001530008052501216。
4. 联系人:富丽娟(计财科科长),电话:024-23986132。
附件1. 第三届“亚洲药剂学论坛”报名回执表
附件2. 会议论文的投稿格式
附件3. 论文模版
沈阳药科大学
<Asian Journal of Pharmaceutical Sciences>编辑部
二零一三年十二月十七日
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ABSTRACT SUMMARY
The format of the abstract is illustrated in this template, designed for the preparation of your abstract. This text is for demonstration only.
Replace the sample title, author listing, author affiliations, designated presenting author’s email address, and abstract text in this template with your information. The ASPST Program Committee recommends that the designated presenting author also be the corresponding author. Use Microsoft Word to prepare your abstract.
Carefully read the instructions and view the Model Abstract before preparing and submitting your abstract.
INTRODUCTION
The format of the abstract is illustrated in this template, designed for the preparation of your abstract. This text is for demonstration only.
Replace the sample title, author listing, author affiliations, designated presenting author’s email address, and abstract text in this template with your information. The ASPST Program Committee recommends that the designated presenting author also be the corresponding author. Use Microsoft Word to prepare your abstract.
Carefully read the instructions and view the Model Abstract before preparing and submitting your abstract.
EXPERIMENTAL METHODS
The format of the abstract is illustrated in this template, designed for the preparation of your abstract.
This text is for demonstration only.
Replace the sample title, author listing, author affiliations, designated presenting author’s email address, and abstract text in this template with your information. The ASPST Program Committee recommends that the designated presenting author also be the corresponding author. Use Microsoft Word to prepare your abstract.
Carefully read the instructions and view the Model Abstract before preparing and submitting your abstract.
RESULTS AND DISCUSSION
The abstract body is in two-column format and must include the following subjects: Abstract Summary, Introduction, Experimental Methods, Results and Discussion, Conclusion, and References. The abstract must include data and tables and/or figures (clearly presented) to support the data. Acknowledgments are optional but recommended.
The abstract body is in two-column format and must include the following subjects: Abstract Summary, Introduction, Experimental Methods, Results and Discussion, Conclusion, and References. The abstract must include data and tables and/or figures (clearly presented) to support the data. Acknowledgments are optional but recommended.
The abstract body is in two-column format and must include the following subjects: Abstract Summary, Introduction, Experimental Methods, Results and Discussion, Conclusion, and References. The abstract must include data and tables and/or figures (clearly presented) to support the data. Acknowledgments are optional but recommended.
The abstract body is in two-column format and must include the following subjects: Abstract Summary, Introduction, Experimental Methods, Results and Discussion, Conclusion, and References. The abstract must include data and tables and/or figures (clearly presented) to support the data. Acknowledgments are optional but recommended.
CONCLUSION
When the abstract is complete and accurate (please proof carefully), convert the abstract to .pdf and save it to a local drive. Only .pdf files will be accepted.
REFERENCES
1. Select “Letter” (8? í 11 inches [21.59 í
2. Accepted font is 10-12 point Arial, Times, Times New Roman, or Helvetica.
3. Title is in bold type and centered across the page.
4. Author names are centered and identified with number superscripts to correspond to author affiliations. Underline the designated presenting author’s name.
5. Author affiliations are centered and identified with number superscripts to correspond to the respective author name; include the designated presenting author’s email address.
6. Abstract body alignment is justified with the first line of each paragraph indented .25 inch (
7. Abstract body format is two-column (3.25 inches [
8. Include one line of space between the title and the name(s) of the author(s) and between author name(s) and affiliation(s).
9. Include one line of space between sections.
10. Abstract must be 2 pages as demonstrated in the Model Abstract.
11. References must be numbered; there are no lines of space between references.
ACKNOWLEDGMENTS
Submitted abstracts must meet the following minimum requirements:
Significant and original contribution within the scope of the Controlled Release Society
Abstract submitted by the deadline
Written in clear English
Few syntax/spelling mistakes
Sufficient data presented, adequately analyzed and discussed with appropriate conclusions supported by the data
Meets format guidelines
Contains data and tables and figures, clearly presented, to support the data
Maximum 2 pages
附件3. 论文模版
Comparison of Methods for Quantifying siRNA Encapsulated into Poly (lactide-co-glycolide) Nanoparticles 2: Novo Nordisk A/S, Novo Nordisk Park B6.2.086, DK-2760
Dongmei Cun1,
1: Department of Pharmaceutics and Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of
ABSTRACT SUMMARY:
Three different strategies to determine siRNA encapsulation efficiency in PLGA nanoparticles were compared. Results show that siRNA can be quantitatively removed from PLGA by extraction. However, the indirect method tends to give false positive results because a considerable part of siRNA was lost during preparation as shown by the direct method and tracking of radio-labeled siRNA.
INTRODUCTION:
The encapsulation efficiency ,of antisense oligonucleotides (ODNs) has been measured after hydrolysis of NPs with 0.5 N NaOH [3]. Shikha et al. [4] used an extraction method applying a buffer/CHCl3 biphasic solvent system followed by UV spectrophotometry to determine the encapsulation efficiency of plasmid DNA. It was proved that the technique could quantitatively and completely remove DNA and preserve the degree of super-coiling of the encapsulated plasmid. However, an indirect method has commonly been employed to evaluate the encapsulation efficiency of DNA since the direct method is often associated with incompletely extraction [5, 6]. In the indirect method, the DNA loading of nanoparticles is determined by subtracting the amount of non-encapsulated DNA from the total amount of DNA. It is difficult to compare literature results obtained with the methods mentioned above due to different preconditions.
In this present study, three different procedures for determination of encapsulation efficiency were compared to assess their applicability for siRNA-loaded PLGA NPs.
EXPERIMENTAL METHODS:
1. Preparation of siRNA-loaded PLGA NPs
SiRNA-loaded NPs were prepared using a double emulsion solvent evaporation technique.
2. Determination of siRNA loading percent and encapsulation efficiency
2.1 NaOH hydrolysis procedure
About 2 mg of NPs were dissolved in 300 μl 0.5 N NaOH under magnetic stirring at RT. The concentration of siRNA in the solution was determined by UV absorbance at 260 nm.
2.2 Extraction procedure
About 2 mg NPs were dissolved in CHCl3 and the CHCl3 layer was extracted four times with TE buffer (pH 7.5). The siRNA concentration in the aqueous phase was determined by the RiboGreen? assay (Molecular probeTM).
2.3 Spiking experiments
Blank PLGA NPs were mixed with a known amount of siRNA, followed by the NaOH hydrolysis or extraction procedure. Recovery (%) was calculated from the ratio of determined concentration to known concentration of siRNA.
2.4 Indirect method
At the last washing step of the NP preparation procedure, the supernatant and washing solutions upon centrifugation were collected and the siRNA content was quantified using the RiboGreen? assay. The encapsulation efficiency was calculated from the difference of the total amount of siRNA added in the formulation and the amount of siRNA in supernatant and washing solution.
3. siRNA mass balance during preparation process
siRNA was 5′-end labelled with (32P)-γ-ATP using T4 polynucleotide kinase. Unlabelled siRNA was removed with a MicroSpin G-25 column (Amersham Biosciences).
The siRNA mass balance during the PLGA NP preparation procedure was determined by liquid scintillation counting.
RESULTS AND DISCUSSION:
1. Comparison of NaOH hydrolysis and extraction
Even though a very good correlation between absorbance value and siRNA concentration (0.5μg/ml~10μg/ml) was obtained, extremely high recovery was obtained in spiking experiments due to the low extinction coefficient of siRNA and the interference of excipients (table 1). The method is not suitable for determining siRNA encapsulation.
Table1. Recovery of different concentration of siRNA after NaOH hydrolysis
|
Added conc. (μg/ml) |
Determined conc. (μg/ml) |
Recovery (%) |
|
0.50 |
6.72±0.12 |
1344±23.8 |
|
2.00 |
4.97±0.10 |
248.3±5.0 |
|
10.00 |
14.02±.0.22 |
140.2±2.2 |
On the contrary, when the extraction procedure was used in stead of solubilization, approximately 100% of added siRNA could be recovered and a single extraction in 60min is sufficient (data not shown). So from the recovery point of view, this method is suitable for determination of siRNA encapsulation efficiency.
2. Comparison of indirect method and direct method (extraction)
The encapsulation efficiency of NPs made from two different PLGAs was measured by the indirect method and the direct extraction method, respectively. The results are shown in table 2. It was observed that siRNA in exterior water phase and siRNA entrapped do not sum up to 100%. There is significant difference between the results of two methods. The similar phenomenon has been observed for DNA [6], due to DNA loss at the interface between CHCl3 and aqueous phase during extraction. However siRNA could be extracted completely. siRNA is therefore lost at another point during the preparation.
Table 2.siRNA encapsulation efficiency (Mean ±s.d, n=3)
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PLGA composition |
75:25 |
50:50 |
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Mw of PLGA |
20 kDa |
80 KDa |
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siRNA in exterior phase (% of initial) |
28.32±4.35 |
20.41±3.39 |
|
Encapsulation efficiency (%)---indirect method |
71.68±4.35 |
79.59±3.39 |
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Encapsulation efficiency (%)---direct method |
5.95±1.72 |
14.20±2.32 |
3. Mass balance of siRNA during preparation
Using radio-labeled siRNA, it was seen that about 34% of total siRNA was lost when the primary emulsion was transferred to exterior water phase due to insufficient emulsification or coalescence. However, a prerequisite of the indirect method is that 100% of added drug exists in the W1/O/W2 system. The result therefore gives a reasonable explanation for the difference between the two methods. In addition, if the lost part of siRNA could be decrease, the encapsulation efficiency will be increased efficiently. So the results indicate that the quality of the primary emulsion is critical for improving encapsulation efficiency.
CONCLUSION:
Under the condition of siRNA solution not being emulsified sufficiently, a considerable part of siRNA was lost when the primary emulsion was transferred to exterior water phase to form double emulsion. Therefore, the indirect method might lead to false positive results. Compared to NaOH hydrolysis and indirect method, the extraction method is suitable for siRNA PLGA NPs. In addition, the quality of primary emulsion is one of the key factors which affect encapsulation efficiency significantly.
REFERENCES:
1. J. Panyam et al. FASEB J. 16 (2002) 1217-1226
2. Y. Xudong et al. J Nanosci Nanotechnol. 6(2006) 2821-2828
3. A.C.Kilic et al. J Microencapsul. 22(2005) 633-641
4.Shikha P. Barman et al. J Control Release. 69 (2006) 337-344
ACKNOWLEDGEMENTS:
Financial support from the Alfred Benzon Foundation (DC) and the Danish Research Council for Technology and Production Sciences (CF) is gratefully acknowledged.
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